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Successful extraction of DNA from 100-year-old herbarium specimens of the liverwort Bazzania trilobata
Authors:
- Kamila Jankowiak,
- Katarzyna Buczkowska,
- Zofia Szweykowska-Kulińska
Abstract
Using a modified DNeasy™ Plant Mini Kit method to extract DNA, we were able to isolate DNA from 100-year-old liverwort herbarium specimens. We sucessfully isolated DNA from 18 specimens of the liverwort Bazzania trilobata collected during the 20th century. Our observations suggest that, at least over 100 years of material storage, the most crucial factor in successful DNA isolation is method of drying, not age of sample.
- Record ID
- UAM1b7d7fe023f94f75b7e808634c4e4113
- Author
- Journal series
- Taxon, ISSN 0040-0262
- Issue year
- 2005
- Vol
- 54
- Pages
- 335-336
- ASJC Classification
- ;
- DOI
- DOI:10.2307/25065361 Opening in a new tab
- Language
- (en) English
- Score (nominal)
- 0
- Score source
- journalList
- Publication indicators
- = 24; = 23; : 2014 = 2.082; : 2006 (2 years) = 2.519 - 2007 (5 years) =2.444
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- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/article/UAM1b7d7fe023f94f75b7e808634c4e4113/
- URN
urn:amu-prod:UAM1b7d7fe023f94f75b7e808634c4e4113
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.