Toward Next-Generation Biohybrid Catalyst Design: Influence of Degree of Polymerization on Enzyme Activity
Authors:
- Marina Kovaliov,
- Thaiesha A. Wright,
- Boyle Cheng,
- Robert T. Mathers,
- Xiangyu Zhang,
- Dong Meng,
- Katarzyna Barbara Szcześniak,
- Jacek Jenczyk,
- Stefan Jurga,
- Devora Cohen-Karni,
- Richard C. Page,
- Dominik Konkolewicz,
- Saadyah Averick
Abstract
Due to their capacity to conduct complex organic transformations, enzymes find extensive use in medical and industrial settings. Unfortunately, enzymes are limited by their poor stability when exposed to harsh non-native conditions. While a host of methods have been developed to stabilize enzymes in non-native conditions, recent research into the synthesis of polymer-enzyme biohybrids using reversible deactivation radical polymerization approaches has demonstrated the potential of increased enzymatic activity in both native and non-native environments. In this manuscript, we utilize the enzyme lipase, as a model system, to explore the impact that modulation of grafted polymer molecular weight has on enzyme activity in both aqueous and organic media. We studied the properties of these hybrids using both solution-phase enzyme activity methods and coarse-grain modeling to assess the impact of polymer grafting density and grafted polymer molecular weight on enzyme activity to gain a deeper insight into this understudied property of the biohybrid system. Copyright © 2020 American Chemical Society.
- Record ID
- UAM1ed26415219c45c7bdccb11c1a9a4982
- Author
- Journal series
- Bioconjugate Chemistry, ISSN 1043-1802, e-ISSN 1520-4812
- Issue year
- 2020
- Vol
- 31
- No
- 3
- Pages
- 939-947
- Keywords in English
- polymer; triacylglycerol lipase, Article; catalyst; chemical structure; density; enzyme activity; hybrid; molecular weight; polymerization; synthesis
- ASJC Classification
- ; ; ; ; ;
- DOI
- DOI:10.1021/acs.bioconjchem.0c00084 Opening in a new tab
- URL
- https://www.scopus.com/inward/record.uri?eid=2-s2.0-85082094512&doi=10.1021%2facs.bioconjchem.0c00084&partnerID=40&md5=3a4c1a1752d36e5878d47d208cf3e0fc Opening in a new tab
- Language
- (en) English
- Score (nominal)
- 100
- Score source
- journalList
- Score
- = 100.0, 13-01-2022, ArticleFromJournal
- Publication indicators
- = 7; = 3; : 2018 = 0.991; : 2019 (2 years) = 4.031 - 2019 (5 years) =4.267
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- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/article/UAM1ed26415219c45c7bdccb11c1a9a4982/
- URN
urn:amu-prod:UAM1ed26415219c45c7bdccb11c1a9a4982
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.