The brome mosaic virus-based recombination vector triggers a limited gene silencing response depending on the orientation of the inserted sequence

Authors:

• Andrzej Pacak,
• Pawel M. Strozycki,
• Maria Monika Barciszewska-Pacak,
• Magdalena Alejska,
• Christophe Lacomme,
• Artur Jarmołowski,
• Zofia Szweykowska-Kulińska,
• Marek Figlerowicz

Abstract

In some RNA viruses (e.g. in brome mosaic virus, BMV), the same factor (intra- or intermolecular hybridization between viral RNA molecules) is capable of inducing two different processes: RNA silencing and RNA recombination. To determine whether there is some interplay between these two phenomena, we have examined if the BMV-based recombination vector containing a plant-genome-derived sequence can function as a gene-silencing vector. Surprisingly, we found that neither dsRNA forming during the replication of the BMV-based vector nor highly structured regions of its genome were effective RNAi triggers. Only mutants carrying a sequence complementary to the target mRNA functioned as gene silencing vectors and were steadily maintained in the infected plant. The constructs containing a sense sequence or inverted repeats did not induce gene silencing but instead were eliminated from the plant cells. © Springer-Verlag 2009.