Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects
Authors:
- David Ballard,
- Jakub Winkler-Galicki,
- Joanna Zofia Wesoły
Abstract
In the last decade, next-generation sequencing (NGS) technology, alternatively massive parallel sequencing (MPS), was applied to all fields of biological research. Its introduction to the field of forensics was slower, mainly due to lack of accredited sequencers, kits, and relatively higher sequencing error rates as compared with standardized Sanger sequencing. Currently, a majority of the problematic issues have been solved, which is proven by the body of reports in the literature. Here, we discuss the utility of NGS sequencing in forensics, emphasizing the advantages, issues, the technical aspects of the experiments, commercial solutions, and the potentially interesting applications of MPS. © 2020, The Author(s).
- Record ID
- UAM47c82818deaf4df7a6abb3e73aaa73f2
- Author
- Journal series
- International Journal of Legal Medicine, ISSN 0937-9827, e-ISSN 1437-1596
- Issue year
- 2020
- Vol
- 134
- No
- 4
- Pages
- 1291-1303
- Publication size in sheets
- 0.60
- Keywords in English
- MPS; Nanopore sequencing; NGS; Nucleic acids; Sequencing by synthesis
- ASJC Classification
- DOI
- DOI:10.1007/s00414-020-02294-0 Opening in a new tab
- URL
- https://www.scopus.com/inward/record.uri?eid=2-s2.0-85086278751&doi=10.1007%2fs00414-020-02294-0&partnerID=40&md5=defaf007d8834b6d2a9a3ef655f44235 Opening in a new tab
- Language
- (en) English
- Score (nominal)
- 100
- Score source
- journalList
- Score
- = 100.0, 17-02-2022, ArticleFromJournal
- Publication indicators
- = 12; : 2018 = 1.069; : 2019 (2 years) = 2.222 - 2019 (5 years) =2.193
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- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/article/UAM47c82818deaf4df7a6abb3e73aaa73f2/
- URN
urn:amu-prod:UAM47c82818deaf4df7a6abb3e73aaa73f2
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.