A circular dichroism study of modified nucleosides
Authors:
- Andrzej Gałat,
- Pawełl Serafinowski,
- Jacek Koput
Abstract
The conformation of 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine was studied by means of circular dichroism in various solvents. In order to calculate the accurate spectral parameters of the Cotton effects, the circular dichroism spectra were resolved into component Gaussian functions which simultaneously fit the adsorption spectra. On the basis of circular dichroism and proton magnetic resonance spectra, these nucleosides were found to occur in the β-configuration with the 3E-gg-anti conformation preferred. Due to the fact that the long-wavelength Cotton effect of mcm5s2U is not masked by the Cotton effects of the other nucleic acid monomers, the molecular parameters of this band may be useful for the conformational analysis of tRNA segments. © 1984.
- Record ID
- UAM73f0b65aa4ad44589bac0964f058330c
- Author
- Journal series
- Biochimica et Biophysica Acta-General Subjects, ISSN 0304-4165
- Issue year
- 1984
- Vol
- 801
- Pages
- 40-47
- ASJC Classification
- ; ;
- DOI
- DOI:10.1016/0304-4165(84)90210-1 Opening in a new tab
- Language
- (en) English
- Score (nominal)
- 0
- Score source
- journalList
- Publication indicators
- = 5; = 5; : 1999 = 0.879; : 2006 (2 years) = 2.024 - 2007 (5 years) =2.704
- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/article/UAM73f0b65aa4ad44589bac0964f058330c/
- URN
urn:amu-prod:UAM73f0b65aa4ad44589bac0964f058330c
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.