In-vitro culture of fertilized embryo sacs of maize: Zygotes and two-celled proembryos can develop into plants
Authors:
- Rafał Mól,
- Elisabeth Matthys-Rochon,
- Christian Dumas
Abstract
Fertilized embryo sacs of Zea mays L. surrounded by a few layers of nucellar cells were cultured in vitro. Primary expiants contained zygotes or twocelled proembryos. Embryos of various sizes and shapes were isolated from 12-48% of explants after two weeks of culture in hormone-free media supplemented with 6-12% of sucrose. Many embryos were at the transition or proembryo stages whilst the rest were either differentiated, with a scutellum, a coleoptile and a shoot apex, or had a deformed apical part. Organogenesis started in 36-89% of embryos cultured on a semisolid medium supplemented with coconut water. Most of the embryos formed only roots but up to 9% of embryos regenerated into plants. This simple method leads the way to plant regeneration from in-vitro-manipulated zygotes or proembryos of maize. © 1993 Springer-Verlag.
- Record ID
- UAMa2bd05c6575e4146a1892610dc1ba6af
- Author
- Journal series
- Planta, ISSN 0032-0935
- Issue year
- 1993
- Vol
- 189
- Pages
- 213-217
- ASJC Classification
- ;
- DOI
- DOI:10.1007/BF00195079 Opening in a new tab
- Language
- (en) English
- Score (nominal)
- 0
- Score source
- journalList
- Publication indicators
- = 38; = 39; : 1999 = 1.285; : 2006 (2 years) = 2.963 - 2007 (5 years) =3.172
- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/article/UAMa2bd05c6575e4146a1892610dc1ba6af/
- URN
urn:amu-prod:UAMa2bd05c6575e4146a1892610dc1ba6af
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.