An efficient 3D cell culture method on biomimetic nanostructured grids.
Authors:
- Maria Wolun-Cholewa,
- Krzysztof Langer,
- Krzysztof Szymanowski,
- Aleksandra Glodek,
- Anna Jankowska,
- Wojciech Warchol,
- Jerzy Langer
Abstract
Current techniques of in vitro cell cultures are able to mimic the in vivo environment only to a limited extent, as they enable cells to grow only in two dimensions. Therefore cell culture approaches should rely on scaffolds that provide support comparable to the extracellular matrix. Here we demonstrate the advantages of novel nanostructured three-dimensional grids fabricated using electro-spinning technique, as scaffolds for cultures of neoplastic cells. The results of the study show that the fibers allow for a dynamic growth of HeLa cells, which form multi-layer structures of symmetrical and spherical character. This indicates that the applied scaffolds are nontoxic and allow proper flow of oxygen, nutrients, and growth factors. In addition, grids have been proven to be useful in in situ examination of cells ultrastructure.
- Record ID
- UAMa35978e4d68f48f49e39b668cae4983c
- Author
- Journal series
- Plos One, ISSN 1932-6203
- Issue year
- 2013
- Vol
- 8
- ASJC Classification
- ; ;
- DOI
- DOI:10.1371/journal.pone.0072936 opening in a new tab
- Language
- en English
- Score (nominal)
- 40
- Score source
- journalList
- Score
- Publication indicators
- = 9; : 2013 = 1.153; : 2013 = 3.534 (2) - 2013=4.015 (5)
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- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/article/UAMa35978e4d68f48f49e39b668cae4983c/
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perishopening in a new tab system.