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Site-specific analysis of protein hydration based on unnatural amino acid fluorescence
Authors:
- Mariana Amaro,
- Jan Brezovsky,
- Silvia Kováčová,
- Jan Sýkora,
- David Bednář,
- Václav Němec,
- Veronika Lišková,
- Nagendra Prasad Kurumbang,
- Koen Beerens,
- Radka Chaloupková,
- Kamil Paruch,
- Martin Hof,
- Jiří Damborský
Abstract
Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations.
- Record ID
- UAMaff1e85e7b6c475e92e146d07966cd22
- Author
- Journal series
- Journal of the American Chemical Society, ISSN 0002-7863
- Issue year
- 2015
- Vol
- 137
- Pages
- 4988-4992
- ASJC Classification
- ; ; ;
- DOI
- DOI:10.1021/jacs.5b01681 Opening in a new tab
- Language
- (en) English
- Score (nominal)
- 45
- Score source
- journalList
- Score
- Publication indicators
- = 18; = 20; : 2015 = 2.600; : 2015 (2 years) = 13.038 - 2015 (5 years) =12.376
- Citation count
- 29
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- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/article/UAMaff1e85e7b6c475e92e146d07966cd22/
- URN
urn:amu-prod:UAMaff1e85e7b6c475e92e146d07966cd22
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.