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EPR study of functionalized magnetite nanoparticles in serum and whole human blood

Tomasz Kubiak

Abstract

The aim of the study was to investigate the properties of the surface-functionalized magnetite nanoparticles in aqueous solutions, serum and human whole blood by means of electron paramagnetic resonance spectroscopy. Five types of magnetite nanoparticles, which differ both in terms of coating (PEG or silane) and attached ligands (doxorubicin or a spin label 4-amino-TEMPO) were used in experiments. EPR measurements proved the impact of the environment on the properties of Fe3O4 nanoparticles. Research showed that the parameters of a broad EPR line from the core of magnetite nanoparticles not only strongly depend on temperature but also vary due to the functionalization of the surface of particles and the medium in which they are dispersed. The use of the Computer Resolution Enhancement Method (CREM) allowed to extract a narrow line with g = 1.99 from a broad EPR signal from the core of nanoparticles. The presence of this narrow component is related to the phenomena occurring on the surface of the core of nanoparticles. The study showed also that the anisotropy of the motion of the spin label 4-amino-TEMPO reflects the properties of the medium and the manner of the attachment of the radical to the surface of nanoparticles. Values of rotational correlation time of spin label were determined for nanoparticles in water, plasma and blood. EasySpin simulations were made for the slow-motional EPR spectra of spin label in low temperature. Moreover, the paramagnetic centers present in serum and whole blood prior to the addition of nanoparticles were identified.
Record ID
UAM0acddd16ca6c46449de7cd8540f5adb4
Diploma type
Doctor of Philosophy
Author
Title in Polish
Badanie metodą EPR funkcjonalizowanych nanocząstek magnetytu w surowicy i pełnej krwi ludzkiej
Title in English
EPR study of functionalized magnetite nanoparticles in serum and whole human blood
Language
pol (pl) Polish
Certifying Unit
Faculty of Physics (SNŚ/WyF/FoP)
Discipline
physics / (physical sciences domain) / (physical sciences)
Scientific discipline (2.0)
6.6 physical sciences
Status
Finished
Defense Date
13-09-2016
Title date
13-09-2016
Supervisor
URL
http://hdl.handle.net/10593/14895 Opening in a new tab
Keywords in English
magnetite nanoparticles, EPR spectroscopy, whole human blood, spin labels, surface functionalization
Abstract in English
The aim of the study was to investigate the properties of the surface-functionalized magnetite nanoparticles in aqueous solutions, serum and human whole blood by means of electron paramagnetic resonance spectroscopy. Five types of magnetite nanoparticles, which differ both in terms of coating (PEG or silane) and attached ligands (doxorubicin or a spin label 4-amino-TEMPO) were used in experiments. EPR measurements proved the impact of the environment on the properties of Fe3O4 nanoparticles. Research showed that the parameters of a broad EPR line from the core of magnetite nanoparticles not only strongly depend on temperature but also vary due to the functionalization of the surface of particles and the medium in which they are dispersed. The use of the Computer Resolution Enhancement Method (CREM) allowed to extract a narrow line with g = 1.99 from a broad EPR signal from the core of nanoparticles. The presence of this narrow component is related to the phenomena occurring on the surface of the core of nanoparticles. The study showed also that the anisotropy of the motion of the spin label 4-amino-TEMPO reflects the properties of the medium and the manner of the attachment of the radical to the surface of nanoparticles. Values of rotational correlation time of spin label were determined for nanoparticles in water, plasma and blood. EasySpin simulations were made for the slow-motional EPR spectra of spin label in low temperature. Moreover, the paramagnetic centers present in serum and whole blood prior to the addition of nanoparticles were identified.
Thesis file
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Uniform Resource Identifier
https://researchportal.amu.edu.pl/info/phd/UAM0acddd16ca6c46449de7cd8540f5adb4/
URN
urn:amu-prod:UAM0acddd16ca6c46449de7cd8540f5adb4

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