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EPR study of functionalized magnetite nanoparticles in serum and whole human blood
Tomasz Kubiak
Abstract
The aim of the study was to investigate the properties of the surface-functionalized magnetite nanoparticles in aqueous solutions, serum and human whole blood by means of electron paramagnetic resonance spectroscopy. Five types of magnetite nanoparticles, which differ both in terms of coating (PEG or silane) and attached ligands (doxorubicin or a spin label 4-amino-TEMPO) were used in experiments. EPR measurements proved the impact of the environment on the properties of Fe3O4 nanoparticles. Research showed that the parameters of a broad EPR line from the core of magnetite nanoparticles not only strongly depend on temperature but also vary due to the functionalization of the surface of particles and the medium in which they are dispersed. The use of the Computer Resolution Enhancement Method (CREM) allowed to extract a narrow line with g = 1.99 from a broad EPR signal from the core of nanoparticles. The presence of this narrow component is related to the phenomena occurring on the surface of the core of nanoparticles. The study showed also that the anisotropy of the motion of the spin label 4-amino-TEMPO reflects the properties of the medium and the manner of the attachment of the radical to the surface of nanoparticles. Values of rotational correlation time of spin label were determined for nanoparticles in water, plasma and blood. EasySpin simulations were made for the slow-motional EPR spectra of spin label in low temperature. Moreover, the paramagnetic centers present in serum and whole blood prior to the addition of nanoparticles were identified.- Record ID
- UAM0acddd16ca6c46449de7cd8540f5adb4
- Diploma type
- Doctor of Philosophy
- Author
- Title in Polish
- Badanie metodą EPR funkcjonalizowanych nanocząstek magnetytu w surowicy i pełnej krwi ludzkiej
- Title in English
- EPR study of functionalized magnetite nanoparticles in serum and whole human blood
- Language
- pol (pl) Polish
- Certifying Unit
- Faculty of Physics (SNŚ/WyF/FoP)
- Discipline
- physics / (physical sciences domain) / (physical sciences)
- Scientific discipline (2.0)
- Status
- Finished
- Defense Date
- 13-09-2016
- Title date
- 13-09-2016
- Supervisor
- URL
- http://hdl.handle.net/10593/14895 Opening in a new tab
- Keywords in English
- magnetite nanoparticles, EPR spectroscopy, whole human blood, spin labels, surface functionalization
- Abstract in English
- The aim of the study was to investigate the properties of the surface-functionalized magnetite nanoparticles in aqueous solutions, serum and human whole blood by means of electron paramagnetic resonance spectroscopy. Five types of magnetite nanoparticles, which differ both in terms of coating (PEG or silane) and attached ligands (doxorubicin or a spin label 4-amino-TEMPO) were used in experiments. EPR measurements proved the impact of the environment on the properties of Fe3O4 nanoparticles. Research showed that the parameters of a broad EPR line from the core of magnetite nanoparticles not only strongly depend on temperature but also vary due to the functionalization of the surface of particles and the medium in which they are dispersed. The use of the Computer Resolution Enhancement Method (CREM) allowed to extract a narrow line with g = 1.99 from a broad EPR signal from the core of nanoparticles. The presence of this narrow component is related to the phenomena occurring on the surface of the core of nanoparticles. The study showed also that the anisotropy of the motion of the spin label 4-amino-TEMPO reflects the properties of the medium and the manner of the attachment of the radical to the surface of nanoparticles. Values of rotational correlation time of spin label were determined for nanoparticles in water, plasma and blood. EasySpin simulations were made for the slow-motional EPR spectra of spin label in low temperature. Moreover, the paramagnetic centers present in serum and whole blood prior to the addition of nanoparticles were identified.
- Thesis file
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- File: 1
- Badanie metodą EPR funkcjonalizowanych nanocząstek magnetytu w surowicy i pełnej krwi ludzkiej, File Tomasz Kubiak praca doktorska.pdf / 3 MB
- Tomasz Kubiak praca doktorska.pdf
- publication date: 20-12-2019
- Badanie metodą EPR funkcjonalizowanych nanocząstek magnetytu w surowicy i pełnej krwi ludzkiej, File Tomasz Kubiak praca doktorska.pdf / 3 MB
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- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/phd/UAM0acddd16ca6c46449de7cd8540f5adb4/
- URN
urn:amu-prod:UAM0acddd16ca6c46449de7cd8540f5adb4