Back
Back
The role of the sequence and structure of mRNAs and anti-sRNAs in their interactions with bacterial noncoding RNAs
Daria Sobańska
Abstract
In the presented PhD dissertation, the role of sequence and structure of mRNAs, and anti-sRNA for their interactions sRNAs was elucidated. So far, the significance of the evolutionally conserved mRNA sequences located in the neighborhood to the sRNA binding sites was not resolved. Little is known about the mode of Hfq action in sRNA interactions with anti-sRNAs. To determine the first issue, S. enterica RybB sRNA interactions with complementary mRNAs containing different nucleotides located 3ʹ to the RybB binding sites were analyzed. To aim the second part of the study, E. coli class I and II sRNA interactions with complementary anti-sRNA and mRNA molecules were compared. The results showed that thermodynamic stability, and the rate of RybB-mRNA complex association were increased, and RybB-dependent ompC mRNA translation repression was more efficient, when purine residues were located 3ʹ to the RybB binding sites in mRNA. It was proposed that the evolutionary preference for purine residues in the mRNA sequences close to the sRNA binding sites enables efficient interactions of those RNAs, which leads to effective mRNA translation repression. Studies concerning second part indicated that RNA binding sites in anti-sRNAs and sRNAs are located in the regions with structure available for binding. sRNA and anti-sRNA molecules specifically interact with Hfq protein. Moreover, it was determined that Hfq-dependent sRNA binding with anti-sRNAs and mRNAs shows similar mechanism, which can be explained by the resemblance of sequences recognized by sRNAs and Hfq in mRNAs and anti-sRNAs.- Record ID
- UAM1cbf995a924a40e29fd208999f630f08
- Diploma type
- Doctor of Philosophy
- Author
- Daria Sobańska (SNP/WB/FoB) Daria Sobańska
- Title in Polish
- Znaczenie sekwencji i struktury mRNA i anty-sRNA w ich odziaływaniach z niekodującymi RNA bakterii
- Title in English
- The role of the sequence and structure of mRNAs and anti-sRNAs in their interactions with bacterial noncoding RNAs
- Language
- pl Polish
- Certifying Unit
- Faculty of Biology (SNP/WB/FoB)
- Discipline
- biochemistry / (biological sciences domain) / (biological sciences)
- Scientific discipline (2.0)
- Defense Date
- 16-04-2018
- End date
- 16-04-2018
- Supervisor
- URL
- http://hdl.handle.net/10593/22728 opening in a new tab
- Keywords in English
- bacterial sRNAs anti-sRNAs
- Abstract in English
- In the presented PhD dissertation, the role of sequence and structure of mRNAs, and anti-sRNA for their interactions sRNAs was elucidated. So far, the significance of the evolutionally conserved mRNA sequences located in the neighborhood to the sRNA binding sites was not resolved. Little is known about the mode of Hfq action in sRNA interactions with anti-sRNAs. To determine the first issue, S. enterica RybB sRNA interactions with complementary mRNAs containing different nucleotides located 3ʹ to the RybB binding sites were analyzed. To aim the second part of the study, E. coli class I and II sRNA interactions with complementary anti-sRNA and mRNA molecules were compared. The results showed that thermodynamic stability, and the rate of RybB-mRNA complex association were increased, and RybB-dependent ompC mRNA translation repression was more efficient, when purine residues were located 3ʹ to the RybB binding sites in mRNA. It was proposed that the evolutionary preference for purine residues in the mRNA sequences close to the sRNA binding sites enables efficient interactions of those RNAs, which leads to effective mRNA translation repression. Studies concerning second part indicated that RNA binding sites in anti-sRNAs and sRNAs are located in the regions with structure available for binding. sRNA and anti-sRNA molecules specifically interact with Hfq protein. Moreover, it was determined that Hfq-dependent sRNA binding with anti-sRNAs and mRNAs shows similar mechanism, which can be explained by the resemblance of sequences recognized by sRNAs and Hfq in mRNAs and anti-sRNAs.
- Thesis file
-
- File: 1
- Znaczenie sekwencji i struktury mRNA i anty-sRNA w ich odziaływaniach z niekodującymi RNA bakterii, File praca doktorska Daria Sobańska.pdf / 6 MB
- praca doktorska Daria Sobańska.pdf
- publication date: 11-12-2019
- Znaczenie sekwencji i struktury mRNA i anty-sRNA w ich odziaływaniach z niekodującymi RNA bakterii, File praca doktorska Daria Sobańska.pdf / 6 MB
-
- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/phd/UAM1cbf995a924a40e29fd208999f630f08/
Back