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The study on amyloidogenesis process in presence of gemini surfactants and with use of microfluidics

Witold Gospodarczyk

Abstract

Amyloid aggregation is a specific type of protein aggregation, in which protein molecules undergo conformational changes and subsequently associate together into characteristic long fibrillar aggregates, called amyloid fibrils. This form of aggregation is responsible for numerous serious human diseases, including Alzheimer disease, Parkinson disease and many other. To make a contribution towards broadening the current knowledge of phenomena underlying amyloid aggregation, an investigation on the influence of microfluidic flow of lysozyme protein and Aβ42 peptide in a chip on the pace of amyloid aggregation was undertaken in this research. Such flow in a channel of small cross section mimics to some extent flow of bodily fluids inside organism vessels; hence, the results of this study were supposed to give the answer whether shear forces occurring during such long-term circulation could be a factor promoting amyloidogenesis. The investigation showed clearly a dramatic increase of pace of amyloid aggregation of examined proteins under microfluidic regime. In this study, the influence of a few selected gemini surfactants, belonging to a very promising class of surfactants, on amyloid aggregation of lysozyme and Aβ42 peptide was also examined. It was revealed that these surfactants inhibited or delayed amyloid aggregation, or segmented already formed fibrils of examined biopolymers.
Record ID
UAMa84e42fca09d4fb7bb7849e98640cca6
Diploma type
Doctor of Philosophy
Author
Witold Gospodarczyk Witold Gospodarczyk,, Undefined Affiliation
Title in Polish
Badania procesu amyloidogenezy w obecności surfaktantów gemini oraz z wykorzystaniem technik mikrofluidycznych
Title in English
The study on amyloidogenesis process in presence of gemini surfactants and with use of microfluidics
Language
en English
Certifying Unit
Faculty of Physics (SNŚ/WyF/FoP)
Discipline
physics / (physical sciences domain) / (physical sciences)
Scientific discipline (2.0)
6.6 physical sciences
Defense Date
19-03-2018
End date
19-03-2018
Supervisor
URL
http://hdl.handle.net/10593/22137 opening in a new tab
Keywords in English
amyloidogenesis, microfluidics, gemini surfactants
Abstract in English
Amyloid aggregation is a specific type of protein aggregation, in which protein molecules undergo conformational changes and subsequently associate together into characteristic long fibrillar aggregates, called amyloid fibrils. This form of aggregation is responsible for numerous serious human diseases, including Alzheimer disease, Parkinson disease and many other. To make a contribution towards broadening the current knowledge of phenomena underlying amyloid aggregation, an investigation on the influence of microfluidic flow of lysozyme protein and Aβ42 peptide in a chip on the pace of amyloid aggregation was undertaken in this research. Such flow in a channel of small cross section mimics to some extent flow of bodily fluids inside organism vessels; hence, the results of this study were supposed to give the answer whether shear forces occurring during such long-term circulation could be a factor promoting amyloidogenesis. The investigation showed clearly a dramatic increase of pace of amyloid aggregation of examined proteins under microfluidic regime. In this study, the influence of a few selected gemini surfactants, belonging to a very promising class of surfactants, on amyloid aggregation of lysozyme and Aβ42 peptide was also examined. It was revealed that these surfactants inhibited or delayed amyloid aggregation, or segmented already formed fibrils of examined biopolymers.

Uniform Resource Identifier
https://researchportal.amu.edu.pl/info/phd/UAMa84e42fca09d4fb7bb7849e98640cca6/

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