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The study on amyloidogenesis process in presence of gemini surfactants and with use of microfluidics
Witold Gospodarczyk
Abstract
Amyloid aggregation is a specific type of protein aggregation, in which protein molecules undergo conformational changes and subsequently associate together into characteristic long fibrillar aggregates, called amyloid fibrils. This form of aggregation is responsible for numerous serious human diseases, including Alzheimer disease, Parkinson disease and many other. To make a contribution towards broadening the current knowledge of phenomena underlying amyloid aggregation, an investigation on the influence of microfluidic flow of lysozyme protein and Aβ42 peptide in a chip on the pace of amyloid aggregation was undertaken in this research. Such flow in a channel of small cross section mimics to some extent flow of bodily fluids inside organism vessels; hence, the results of this study were supposed to give the answer whether shear forces occurring during such long-term circulation could be a factor promoting amyloidogenesis. The investigation showed clearly a dramatic increase of pace of amyloid aggregation of examined proteins under microfluidic regime. In this study, the influence of a few selected gemini surfactants, belonging to a very promising class of surfactants, on amyloid aggregation of lysozyme and Aβ42 peptide was also examined. It was revealed that these surfactants inhibited or delayed amyloid aggregation, or segmented already formed fibrils of examined biopolymers.- Record ID
- UAMa84e42fca09d4fb7bb7849e98640cca6
- Diploma type
- Doctor of Philosophy
- Author
- Witold Gospodarczyk Witold Gospodarczyk
- Title in Polish
- Badania procesu amyloidogenezy w obecności surfaktantów gemini oraz z wykorzystaniem technik mikrofluidycznych
- Title in English
- The study on amyloidogenesis process in presence of gemini surfactants and with use of microfluidics
- Language
- en English
- Certifying Unit
- Faculty of Physics (SNŚ/WyF/FoP)
- Discipline
- physics / (physical sciences domain) / (physical sciences)
- Scientific discipline (2.0)
- Defense Date
- 19-03-2018
- End date
- 19-03-2018
- Supervisor
- URL
- http://hdl.handle.net/10593/22137 opening in a new tab
- Keywords in English
- amyloidogenesis, microfluidics, gemini surfactants
- Abstract in English
- Amyloid aggregation is a specific type of protein aggregation, in which protein molecules undergo conformational changes and subsequently associate together into characteristic long fibrillar aggregates, called amyloid fibrils. This form of aggregation is responsible for numerous serious human diseases, including Alzheimer disease, Parkinson disease and many other. To make a contribution towards broadening the current knowledge of phenomena underlying amyloid aggregation, an investigation on the influence of microfluidic flow of lysozyme protein and Aβ42 peptide in a chip on the pace of amyloid aggregation was undertaken in this research. Such flow in a channel of small cross section mimics to some extent flow of bodily fluids inside organism vessels; hence, the results of this study were supposed to give the answer whether shear forces occurring during such long-term circulation could be a factor promoting amyloidogenesis. The investigation showed clearly a dramatic increase of pace of amyloid aggregation of examined proteins under microfluidic regime. In this study, the influence of a few selected gemini surfactants, belonging to a very promising class of surfactants, on amyloid aggregation of lysozyme and Aβ42 peptide was also examined. It was revealed that these surfactants inhibited or delayed amyloid aggregation, or segmented already formed fibrils of examined biopolymers.
- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/phd/UAMa84e42fca09d4fb7bb7849e98640cca6/
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