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Structural and spectroscopic studies of complexes of bis-imidazolium quaternary salts with lipids and nucleic acids

Wojciech Kida

Abstract

To be considered for medical applications, drug delivery systems are required to be effective and nontoxic. Conventionally, such systems are composed of therapeutic substances (drug molecules, proteins, genes) encapsulated within a carrier. When dealing with genes, many macromolecules like viruses, polymers and lipids have already been tested as potential carriers but recently, a diverse group of mixtures of lipids and surfactants have appeared to be a promising type of carrier for nonviral gene delivery. The aim of this study was to analyse the influence of four gemini surfactantson the structure and phase behaviour of aqueous solutions of phospholipid (DPPC) and afterwards to determine the possibility of using these mixed systems as vehicles for gene therapy. These systems were characterised using small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) Fourier transform infrared spectroscopy (FTIR), circular dichroism spectroscopy (CD) and electrophoretic tests. At the end cytotoxicity test were conducted. The results of performed measurements have allowed us to assess the connection between the geometry of gemini surfactant and structures formed by two- and three-component systems. Moreover, the tested group of gemini surfactants with short acyl chain decrease the temperature of main phase transition whereas the gemini surfactants with long acyl chain increase it. Results of complexation of nucleic acids showed that the most effective gemini surfactant was surfactant with longer spacer and hexadecylo- side chains.
Record ID
UAMf441670d12ea4ff1ab78c85ce48ca6a9
Diploma type
Doctor of Philosophy
Author
Wojciech Kida (SNŚ/WyF/FoP) Wojciech Kida,,
Title in Polish
Badania strukturalne i spektroskopowe kompleksów czwartorzędowych soli bis-imidazoliowych z lipidami i kwasami nukleinowymi
Title in English
Structural and spectroscopic studies of complexes of bis-imidazolium quaternary salts with lipids and nucleic acids
Language
pl Polish
Certifying Unit
Faculty of Physics (SNŚ/WyF/FoP)
Discipline
physics / (physical sciences domain) / (physical sciences)
Scientific discipline (2.0)
6.6 physical sciences
Defense Date
04-02-2016
End date
04-02-2016
Supervisor
URL
http://hdl.handle.net/10593/14267 opening in a new tab
Keywords in English
gemini Surfactants, lipoplexes,gene therapy
Abstract in English
To be considered for medical applications, drug delivery systems are required to be effective and nontoxic. Conventionally, such systems are composed of therapeutic substances (drug molecules, proteins, genes) encapsulated within a carrier. When dealing with genes, many macromolecules like viruses, polymers and lipids have already been tested as potential carriers but recently, a diverse group of mixtures of lipids and surfactants have appeared to be a promising type of carrier for nonviral gene delivery. The aim of this study was to analyse the influence of four gemini surfactantson the structure and phase behaviour of aqueous solutions of phospholipid (DPPC) and afterwards to determine the possibility of using these mixed systems as vehicles for gene therapy. These systems were characterised using small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) Fourier transform infrared spectroscopy (FTIR), circular dichroism spectroscopy (CD) and electrophoretic tests. At the end cytotoxicity test were conducted. The results of performed measurements have allowed us to assess the connection between the geometry of gemini surfactant and structures formed by two- and three-component systems. Moreover, the tested group of gemini surfactants with short acyl chain decrease the temperature of main phase transition whereas the gemini surfactants with long acyl chain increase it. Results of complexation of nucleic acids showed that the most effective gemini surfactant was surfactant with longer spacer and hexadecylo- side chains.

Uniform Resource Identifier
https://researchportal.amu.edu.pl/info/phd/UAMf441670d12ea4ff1ab78c85ce48ca6a9/

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