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Structural and spectroscopic studies of complexes of bis-imidazolium quaternary salts with lipids and nucleic acids
Wojciech Kida
Abstract
To be considered for medical applications, drug delivery systems are required to be effective and nontoxic. Conventionally, such systems are composed of therapeutic substances (drug molecules, proteins, genes) encapsulated within a carrier. When dealing with genes, many macromolecules like viruses, polymers and lipids have already been tested as potential carriers but recently, a diverse group of mixtures of lipids and surfactants have appeared to be a promising type of carrier for nonviral gene delivery. The aim of this study was to analyse the influence of four gemini surfactantson the structure and phase behaviour of aqueous solutions of phospholipid (DPPC) and afterwards to determine the possibility of using these mixed systems as vehicles for gene therapy. These systems were characterised using small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) Fourier transform infrared spectroscopy (FTIR), circular dichroism spectroscopy (CD) and electrophoretic tests. At the end cytotoxicity test were conducted. The results of performed measurements have allowed us to assess the connection between the geometry of gemini surfactant and structures formed by two- and three-component systems. Moreover, the tested group of gemini surfactants with short acyl chain decrease the temperature of main phase transition whereas the gemini surfactants with long acyl chain increase it. Results of complexation of nucleic acids showed that the most effective gemini surfactant was surfactant with longer spacer and hexadecylo- side chains.- Record ID
- UAMf441670d12ea4ff1ab78c85ce48ca6a9
- Diploma type
- Doctor of Philosophy
- Author
- Wojciech Kida (SNŚ/WyF/FoP) Wojciech Kida
- Title in Polish
- Badania strukturalne i spektroskopowe kompleksów czwartorzędowych soli bis-imidazoliowych z lipidami i kwasami nukleinowymi
- Title in English
- Structural and spectroscopic studies of complexes of bis-imidazolium quaternary salts with lipids and nucleic acids
- Language
- pl Polish
- Certifying Unit
- Faculty of Physics (SNŚ/WyF/FoP)
- Discipline
- physics / (physical sciences domain) / (physical sciences)
- Scientific discipline (2.0)
- Defense Date
- 04-02-2016
- End date
- 04-02-2016
- Supervisor
- URL
- http://hdl.handle.net/10593/14267 opening in a new tab
- Keywords in English
- gemini Surfactants, lipoplexes,gene therapy
- Abstract in English
- To be considered for medical applications, drug delivery systems are required to be effective and nontoxic. Conventionally, such systems are composed of therapeutic substances (drug molecules, proteins, genes) encapsulated within a carrier. When dealing with genes, many macromolecules like viruses, polymers and lipids have already been tested as potential carriers but recently, a diverse group of mixtures of lipids and surfactants have appeared to be a promising type of carrier for nonviral gene delivery. The aim of this study was to analyse the influence of four gemini surfactantson the structure and phase behaviour of aqueous solutions of phospholipid (DPPC) and afterwards to determine the possibility of using these mixed systems as vehicles for gene therapy. These systems were characterised using small angle X-ray scattering (SAXS), differential scanning calorimetry (DSC) Fourier transform infrared spectroscopy (FTIR), circular dichroism spectroscopy (CD) and electrophoretic tests. At the end cytotoxicity test were conducted. The results of performed measurements have allowed us to assess the connection between the geometry of gemini surfactant and structures formed by two- and three-component systems. Moreover, the tested group of gemini surfactants with short acyl chain decrease the temperature of main phase transition whereas the gemini surfactants with long acyl chain increase it. Results of complexation of nucleic acids showed that the most effective gemini surfactant was surfactant with longer spacer and hexadecylo- side chains.
- Uniform Resource Identifier
- https://researchportal.amu.edu.pl/info/phd/UAMf441670d12ea4ff1ab78c85ce48ca6a9/
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